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MALDI mass spectrometry imaging of the three experimental groups. ( A ) Glutathione (GSH) at m / z 306.0768 [M-H] − , obtained <t>by</t> <t>met-ID</t> and confirmed from MSMS-fragmentation ( p = 0.032); ( B ) glutathione (GSSG) at m / z 611.1440 [M-H] − , identified based on accurate mass ( p = 0.2443); ( C ) cholesterol sulphate at m / z 465.3049 [M-H] − ( p = 0.050); and ( D ) sphingomyelin (33:1) at m / z 687.5452 [M-H] − ( p = 0.009), obtained by met-ID, respectively. Coloration of the respective group regions is given at the bottom of the figure. AS: angII + semaglutide; AV: angII + vehicle; SV: saline + vehicle. Box-plots and p -values were obtained from one-way ANOVA of the three groups.
In House Developed Software Met Id, supplied by Human Metabolome Technologies America, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MALDI mass spectrometry imaging of the three experimental groups. ( A ) Glutathione (GSH) at m / z 306.0768 [M-H] − , obtained <t>by</t> <t>met-ID</t> and confirmed from MSMS-fragmentation ( p = 0.032); ( B ) glutathione (GSSG) at m / z 611.1440 [M-H] − , identified based on accurate mass ( p = 0.2443); ( C ) cholesterol sulphate at m / z 465.3049 [M-H] − ( p = 0.050); and ( D ) sphingomyelin (33:1) at m / z 687.5452 [M-H] − ( p = 0.009), obtained by met-ID, respectively. Coloration of the respective group regions is given at the bottom of the figure. AS: angII + semaglutide; AV: angII + vehicle; SV: saline + vehicle. Box-plots and p -values were obtained from one-way ANOVA of the three groups.
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MALDI mass spectrometry imaging of the three experimental groups. ( A ) Glutathione (GSH) at m / z 306.0768 [M-H] − , obtained <t>by</t> <t>met-ID</t> and confirmed from MSMS-fragmentation ( p = 0.032); ( B ) glutathione (GSSG) at m / z 611.1440 [M-H] − , identified based on accurate mass ( p = 0.2443); ( C ) cholesterol sulphate at m / z 465.3049 [M-H] − ( p = 0.050); and ( D ) sphingomyelin (33:1) at m / z 687.5452 [M-H] − ( p = 0.009), obtained by met-ID, respectively. Coloration of the respective group regions is given at the bottom of the figure. AS: angII + semaglutide; AV: angII + vehicle; SV: saline + vehicle. Box-plots and p -values were obtained from one-way ANOVA of the three groups.
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MALDI mass spectrometry imaging of the three experimental groups. ( A ) Glutathione (GSH) at m / z 306.0768 [M-H] − , obtained <t>by</t> <t>met-ID</t> and confirmed from MSMS-fragmentation ( p = 0.032); ( B ) glutathione (GSSG) at m / z 611.1440 [M-H] − , identified based on accurate mass ( p = 0.2443); ( C ) cholesterol sulphate at m / z 465.3049 [M-H] − ( p = 0.050); and ( D ) sphingomyelin (33:1) at m / z 687.5452 [M-H] − ( p = 0.009), obtained by met-ID, respectively. Coloration of the respective group regions is given at the bottom of the figure. AS: angII + semaglutide; AV: angII + vehicle; SV: saline + vehicle. Box-plots and p -values were obtained from one-way ANOVA of the three groups.
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MALDI mass spectrometry imaging of the three experimental groups. ( A ) Glutathione (GSH) at m / z 306.0768 [M-H] − , obtained <t>by</t> <t>met-ID</t> and confirmed from MSMS-fragmentation ( p = 0.032); ( B ) glutathione (GSSG) at m / z 611.1440 [M-H] − , identified based on accurate mass ( p = 0.2443); ( C ) cholesterol sulphate at m / z 465.3049 [M-H] − ( p = 0.050); and ( D ) sphingomyelin (33:1) at m / z 687.5452 [M-H] − ( p = 0.009), obtained by met-ID, respectively. Coloration of the respective group regions is given at the bottom of the figure. AS: angII + semaglutide; AV: angII + vehicle; SV: saline + vehicle. Box-plots and p -values were obtained from one-way ANOVA of the three groups.
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MALDI mass spectrometry imaging of the three experimental groups. ( A ) Glutathione (GSH) at m / z 306.0768 [M-H] − , obtained <t>by</t> <t>met-ID</t> and confirmed from MSMS-fragmentation ( p = 0.032); ( B ) glutathione (GSSG) at m / z 611.1440 [M-H] − , identified based on accurate mass ( p = 0.2443); ( C ) cholesterol sulphate at m / z 465.3049 [M-H] − ( p = 0.050); and ( D ) sphingomyelin (33:1) at m / z 687.5452 [M-H] − ( p = 0.009), obtained by met-ID, respectively. Coloration of the respective group regions is given at the bottom of the figure. AS: angII + semaglutide; AV: angII + vehicle; SV: saline + vehicle. Box-plots and p -values were obtained from one-way ANOVA of the three groups.
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MALDI mass spectrometry imaging of the three experimental groups. ( A ) Glutathione (GSH) at m / z 306.0768 [M-H] − , obtained <t>by</t> <t>met-ID</t> and confirmed from MSMS-fragmentation ( p = 0.032); ( B ) glutathione (GSSG) at m / z 611.1440 [M-H] − , identified based on accurate mass ( p = 0.2443); ( C ) cholesterol sulphate at m / z 465.3049 [M-H] − ( p = 0.050); and ( D ) sphingomyelin (33:1) at m / z 687.5452 [M-H] − ( p = 0.009), obtained by met-ID, respectively. Coloration of the respective group regions is given at the bottom of the figure. AS: angII + semaglutide; AV: angII + vehicle; SV: saline + vehicle. Box-plots and p -values were obtained from one-way ANOVA of the three groups.
Self Developed Imap Software, supplied by Metabo Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


MALDI mass spectrometry imaging of the three experimental groups. ( A ) Glutathione (GSH) at m / z 306.0768 [M-H] − , obtained by met-ID and confirmed from MSMS-fragmentation ( p = 0.032); ( B ) glutathione (GSSG) at m / z 611.1440 [M-H] − , identified based on accurate mass ( p = 0.2443); ( C ) cholesterol sulphate at m / z 465.3049 [M-H] − ( p = 0.050); and ( D ) sphingomyelin (33:1) at m / z 687.5452 [M-H] − ( p = 0.009), obtained by met-ID, respectively. Coloration of the respective group regions is given at the bottom of the figure. AS: angII + semaglutide; AV: angII + vehicle; SV: saline + vehicle. Box-plots and p -values were obtained from one-way ANOVA of the three groups.

Journal: Biomedicines

Article Title: Semaglutide Prevents Aortic Rupture and Dissection in the Angiotensin II Mouse Model

doi: 10.3390/biomedicines14040933

Figure Lengend Snippet: MALDI mass spectrometry imaging of the three experimental groups. ( A ) Glutathione (GSH) at m / z 306.0768 [M-H] − , obtained by met-ID and confirmed from MSMS-fragmentation ( p = 0.032); ( B ) glutathione (GSSG) at m / z 611.1440 [M-H] − , identified based on accurate mass ( p = 0.2443); ( C ) cholesterol sulphate at m / z 465.3049 [M-H] − ( p = 0.050); and ( D ) sphingomyelin (33:1) at m / z 687.5452 [M-H] − ( p = 0.009), obtained by met-ID, respectively. Coloration of the respective group regions is given at the bottom of the figure. AS: angII + semaglutide; AV: angII + vehicle; SV: saline + vehicle. Box-plots and p -values were obtained from one-way ANOVA of the three groups.

Article Snippet: Peak m / z recalibration and preliminary metabolite identification were performed by using accurate mass measurements and the in-house developed software Met-ID (version 0.5.8) [ ] which includes searchable metabolites entries from the Human Metabolome Database (HMDB) [ ] and LIPID MAPS [ , ].

Techniques: Mass Spectrometry, Imaging, Saline

MALDI mass spectrometry imaging and box-plot of m / z 734.5348 (corresponding to the m / z of galactosylceramide (d18:1/16:0) [M+Cl]), obtained by met-ID, of the angII + semaglutide (orange) vs. the agII + vehicle (red) groups. The very low intensities in the saline + vehicle group (green) are also included in the figure for comparison.

Journal: Biomedicines

Article Title: Semaglutide Prevents Aortic Rupture and Dissection in the Angiotensin II Mouse Model

doi: 10.3390/biomedicines14040933

Figure Lengend Snippet: MALDI mass spectrometry imaging and box-plot of m / z 734.5348 (corresponding to the m / z of galactosylceramide (d18:1/16:0) [M+Cl]), obtained by met-ID, of the angII + semaglutide (orange) vs. the agII + vehicle (red) groups. The very low intensities in the saline + vehicle group (green) are also included in the figure for comparison.

Article Snippet: Peak m / z recalibration and preliminary metabolite identification were performed by using accurate mass measurements and the in-house developed software Met-ID (version 0.5.8) [ ] which includes searchable metabolites entries from the Human Metabolome Database (HMDB) [ ] and LIPID MAPS [ , ].

Techniques: Mass Spectrometry, Imaging, Saline, Comparison